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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: DNA Methylation Analysis Identifies Novel Epigenetic Loci in Dilated Murine Heart upon Exposure to Volume Overload
doi: 10.3390/ijms24065885
Figure Lengend Snippet: Long-term VO (16 weeks post-shunt) resulted in pathological cardiac remodeling and HF progression. ( A ) Workflow for DNA methylation analysis in VO mice. ( B – D ) Echocardiographic parameters: HR, heart rate ( B ); LVEDD, LV end-diastolic diameter ( C ); EF, ejection fraction ( D ). ( E ) Kaplan–Meier survival analysis, log-rank test, n = 9 sham and 19 shunt mice. ( F , G ) Morphometric parameters: LVW/TL, LV weight-to-tibia length ( F ); LungW/TL, lung weight-to-tibia length ( G ). ( H ) Representative images of WGA- (upper panels) and picrosirius red- (lower panels) stained myocardial cross-sections. ( I ) Quantitative real time PCR analysis for the expression of fetal cardiac stress genes. Values are mean ± SEM, n = 3–6 hearts/group. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. sham, two-tailed unpaired Student’s t -test.
Article Snippet: Cardiac tissues were fixed in 4% formalin, embedded in paraffin sections (6 μm), and stained with either fluorescein-conjugated wheat germ agglutinin (WGA-Alexa Fluor 594, Invitrogen, Carlsbad, CA, USA) for cross-sectional area assessment or
Techniques: DNA Methylation Assay, Staining, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test